Molecular Biology/Plasmid Constructs Co-Op

<div class="content-intro"><p style="line-height: 1.2;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases. Headquartered in Somerset, New Jersey, we are developing advanced cell therapies across a diverse array of technology platforms, including autologous and allogenic chimeric antigen receptor T-cell, T-cell receptor (TCR-T), and natural killer (NK) cell-based immunotherapy. From our three RD sites around the world, we apply these innovative technologies to pursue the discovery of safe, efficacious and cutting-edge therapeutics for patients worldwide.</span></p> <p style="line-height: 1.2;"> </p> <p style="line-height: 1.2;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Legend Biotech entered into a global collaboration agreement with Janssen, one of the pharmaceutical companies of Johnson Johnson, to jointly develop and commercialize ciltacabtagene autolecuel (cilta-cel). Our strategic partnership is designed to combine the strengths and expertise of both companies to advance the promise of an immunotherapy in the treatment of multiple myeloma.</span></p> <p style="line-height: 1.2;"> </p></div><p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;">Legend Biotech is seeking a <strong>Molecular Biology/Plasmid Constructs Co-Op</strong> as part of the <strong>RD </strong>team based in <strong>Philadelphia, PA</strong>.</span></p> <p> </p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;"><strong>Role Overview</strong></span></p> <p> </p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">The Molecular Biology/Plasmid Constructs Co-Op will contribute to the design and functional validation of synthetic and natural promoters for T-cell gene expression, leveraging bioinformatics tools and molecular biology techniques. This role combines computational analysis with hands-on lab work to advance gene regulation strategies in cutting-edge gene therapy research.</span></p> <p><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><strong>Co-Op Duration</strong>: 6-month (Summer to Fall) with possible extension.</span></p> <p> </p> <p><span style="font-size: 12pt; font-family: arial, helvetica, sans-serif;"><strong>Key Responsibilities</strong></span></p> <ul> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Assist in designing synthetic and natural promoters for T-cell specific gene expression using bioinformatics tools and literature mining.</span></li> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Perform cloning of promoter sequences into lentiviral vectors using standard molecular biology techniques (PCR, restriction digestion, ligation, Gibson assembly).</span></li> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Maintain mammalian cell lines and primary T cells; support transfection and transduction experiments.</span></li> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Conduct reporter assays (e.g. luciferase, GFP) to evaluate promoter strength and specificity in T cells or cell lines.</span></li> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; font-size: 12pt;">Analyze experimental data using software such as GraphPad Prism, Excel, and basic statistical methods.</span></li> <li style="font-family: arial, helvetica, sans-serif; font-size: 12pt;"><span style="font-family: arial, helvetica, sans-serif; fo ... (truncated, view full listing at source)